Get A Laboratory Guide to Glycoconjugate Analysis PDF

By Uwe D. Neue, Charles H. Phoebe (auth.), Dr. Peter Jackson, Prof. John T. Gallagher (eds.)

ISBN-10: 3034873883

ISBN-13: 9783034873888

ISBN-10: 3034873905

ISBN-13: 9783034873901

18. 2 precept of FACE/Gel Retardation Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 18. three Labelling of Oligosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. four Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. five size of Binding consistent for the interplay among Protein and ANTS-Labelled Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355 18. 6 dimension of Binding consistent for the interplay among Protein and local Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360 ~ the applying of Capillary Affinity Electrophoresis to the research _ of Carbohydrate-Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 1 advent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 2 precept of CAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 19. three decision of organization Constants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 364 19. four Technical methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 normal concerns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 19. five barriers of the approach . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 370 19. 6 software of CAE to the research of Carbohydrate-Protein Interactions . . . . . . 371 19. 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377 20. 1 advent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 380 20. 2 Technical techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381 20. three pattern Detection and pattern restoration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Autoradiography and marking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 pattern detection by means of blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Semipreparative ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390 20. four research of information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Measuring pattern mobilities - calculating a retardation coefficient . . . . . . . . . . . . 391 Graphical research of information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 392 analyzing ACE styles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 393 opposite ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395 20. five precis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 topic Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399 XII record of individuals Nebojsa Avdalovic John T. Gallagher Dionex company melanoma learn crusade division of clinical Oncology 445 Lakeside force collage of Manchester Sunnyvale, CA 94086 Christie CRC learn Centre Klaus Biemann Wilmslow street division of Chemistry Manchester M20 4BX Massachusetts Institute of expertise united kingdom Cambridge, MA 02139-4307 united states Geoffrey R.

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One observes a sudden loss in retention, and the column needs to be rewetted with an organic solvent to regenerate retention. g. Resolve CI8 and Dextro-Pak from Waters) (Fig. 4). RP chromatography is also ideal as a micropreparative tool. The solubility of the analytes in the mobile phase (100% water) is excellent, and no additional cleanup steps are needed after the chromatographic step. Also, large-diameter columns are readily available if the preparation needs to be scaled up to larger amounts.

106: 238 68 Honda S, Matsuda Y, Takahashi M, Kakehi K and Ganno S (1980) Anal. Chern. 52: 1079 69 Honda S, Takahashi M, Kakehi K and Ganno S (1981)Anal. Biochern. 113: 130 22 70 Mopper K, Dawson R, Liebezeit G and 76 Thomas J and MortAJ (1994) Anal. Biochem. 223: 99 77 Lampio A and Finne J (1991) Anal. Biochern. 197: 132 78 Maes PC, Nagels LJ and Spanoghe BR (1993) Chrornatographia 37: 511 79 Maes PC, Nagels L, Dewaele C and Alderweireldt FC (1991) 1. Chrornatogr. 558: 343 80 Wee tall HH (1976) Methods Enzyrnol.

G. g. following SDS-PAGE (10), Western blotting (11, 12) or dot blotting. g. PhastTransfer™ Pharmacia). g. PVDF, nitrocellulose and nylon, may be used. Although the streptavidin-alkaline phosphatase detection system will function with nylon membranes, high backgrounds are observed. PVDF and nitrocellulose membranes are better, if they are available, as they show little or no background staining. g. Immobilon pTM from Millipore). re Samples bound to the membrane either by dot blotting or by 50S-PAGE followed by Western blotting.

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A Laboratory Guide to Glycoconjugate Analysis by Uwe D. Neue, Charles H. Phoebe (auth.), Dr. Peter Jackson, Prof. John T. Gallagher (eds.)


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